The UMGC provides digital PCR through the QX200 Droplet Digital PCR (ddPCR) system produced by Bio-Rad. This technology offers flexible digital PCR chemistry that can use Taqman hydrolysis probes or EvaGreen assays for high-precision absolute quantification of nucleic acid targets without the need for a standard curve. Digital PCR is ideal for measurements of copy number variation, rare sequence detection, gene expression, target DNA/RNA, and microbial quantification.
Bio-Rad Droplet Digital PCR
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Quantasoft, s.r.o. U Pergamenky 1145/12 170 00 Praha 7 Czech Republic Org. ID: 27564827 VAT ID: CZ27564827. [email protected] +465. QuantaSoft Analysis Pro Instruction Manual 3 QuantaSoft™ Analysis Pro (AP) software is available to download and install from www.bio-rad.com. There is no installation disc provided for the software. QuantaSoft Standard Edition is still required to operate the QX100 or QX200 Droplet Reader.
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We offer two ddPCR service options to serve our University of Minnesota clients with additional services to be added later this year. Our current service options are client operated to give users ultimate flexibility with experimental design and project turnaround.
Clients purchase their own Bio-Rad reagents and consumables, including the EvaGreen Supermix or the Supermix for Probes, and use a dedicated ddPCR workstation in NHH to complete the following QX200 workflow:
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up ddPCR experiments using the Bio-Rad system.
Please note, one-time instrument training is required for all new users before using the ddPCR workstation.
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Please contact Darrell Johnson at [email protected] for further details on the QX200 ddPCR system.
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